IAM Drug Discovery Phases

For membrane protein purification and drug membrane permeability prediction

The IAM Drug Discovery HPLC column is an ideal tool for high throughput predidiction of drug membrane permeabslity. Phosphatldylcholine (PC) is the malor phospholipid found in cell membranes. lAM chromatography phases prepared from PC analogs closely mimic the surface of a biological cell membrane. Consequently, IAM phases display a high affinity for membrane proteins and are useful in membrane protein purification and in the study of drug-membrane interactions.

The IAM surface is formed by covalently bonding the membrane-forming phospholipids to silica (Figure 1). The results from the IAM column correlate well to traditional in vitro methods such as intestinal tissue and Caco-2 Cells, yet are faster and cheaper to achieve.

Other phases such as ODS silica, for example, retains analytes solely on the basis of hydrophobicity. 1AM more closely mimics the interaction of analytes with biological membranes, where a combination of hydrophobic, ion pairing and hydrogen bonding interactions are possible. This combination of interactions measured by the IAM column is known as phospholipophilicity. These advances have led to the development of several new IAM phases used for prediding drug membrane permeability-the IAM.PC.DD2 and the IAM Fast-screen Mini Column.

Products

Several different types of IAM columns are used for various applications:

IAM.PC IAM.PC.MG	Membrane Protein Purification
IAM.PC.DD2	Drug Discovery - for drug membrane permeability prediction that are hydrophobic in nature.
IAM Fast-Screen Mini Columns	High throiughput estimation of drug permeability

Note: The lAM DD column has been withdrawn from the market. Current users are asked to migrate to the IAM DD2 column.