Chiral AGP
A chiral HPLC-column for direct resolution of enantiomers
The chiral selector in the CHIRAL-AGP phase is alpha1-acid glycoprotein (AGP). This very stable protein has been immobilized onto spherical 5 micrometer silica particles. The protein tolerates high concentrations of organic solvents, high and low pH, and high temperatures. The column is used in the reversed-phase and can be used for direct resolution of enantiomers, without derivatization. The CHIRAL-AGP column separates enantiomers of an extremely broad range of drug compounds:
- amines (primary, secondary, tertiary, and quaternary)
- acids (strong and weak)
- nonprotolytes (amides, esters, alcohols, sulphoxides, etc.)
CHIRAL-AGP is most likely the column with the broadest applicability of all chiral columns available today.
Application Areas
At pharmaceutical companies, hospitals, universities and chemical industry CHIRAL-AGP is used for the analysis of enantiomeric purity and for bioanalysis. A growing application area, due to the exceptional applicability of the phase, is isolation of pure enantiomers on semipreparative columns.
Mobile Phases
Very simple mobile phase systems are used for the majority of the separations; buffered waterbased mobile phases with the addition of low concentrations of an organic modifier, such as 2-propanol or acetonitrile for example. The changes affect both the solute and the protein on the stationary phase. The changes affect different types of solutes in a different ways. Most of the separations (>95%) have been performed using the above described simple mobile phases. However, in some cases charged modifiers as DMOA (N,N-dimethyloctylamine), octanic acid and quaternary ammonium compounds are used to induce enantioselectivity.
| Product | Particle Size | Column Dimensions | Regis
Product # | U.S. Price |
| Spherical Silica |
| Chiral AGP | 5 µm | 1 cm x 3.0 mm i.d. guard | 732300 | $170.00 |  |
| Chiral AGP | 5 µm | 10 cm x 2.0 mm i.d. | 732196 | $1,775.00 | |
| Chiral AGP | 5 µm | 10 cm x 4.0 mm i.d. | 732200 | $1,650.00 | |
| Chiral AGP | 5 µm | 15 cm x 2.0 mm i.d. | 732197 | $2,200.00 | |
| Chiral AGP | 5 µm | 15 cm x 4.0 mm i.d. | 732199 | $2,100.00 | |
| Chiral AGP | 5 µm | 5 cm x 2.0 mm i.d. | 732201 | $1,400.00 | |
| Chiral AGP | 5 µm | 5 cm x 4.0 mm i.d. | 732198 | $1,275.00 | |