Coelenterazine is the substrate that illuminates many invertebrate marine animals such as the luminescent Aequorea jellyfish. Like firefly luciferin, coelenterazine can be used in:
- Gene reporter assays
- Whole animal assays
- Biochemical assays (i.e., ELISA and Bioluminescence Resonance Energy Transfer [BRET]).
- Live-cell assays for the detection of calcium ions
The Coelenterazine Reaction:
When coelenterazine combines with luciferase, oxygen, and calcium ions, it produces oxy-coelenterazine and light. This allows coelenterazine to be used as a detection reagent for luciferase, oxygen, or calcium. Unlike fluorescence, the detection of bioluminescence in diagnostic tests can be performed in the dark thereby eliminating background noise.
Affordable Coelenterazine in a Range of Convenient Quantities
Coelenterazine (native or H) is available from Regis' online store in two prepack sizes.
Why Order Coelenterazine from Regis?
We synthesize coelenterazine at our GMP laboratory in suburban Chicago. This way, you know you will receive a high quality reagent that is reproducible time after time. We manufacture the highest purity luciferin and coelenterazine in the industry, and we test it for QC by HPLC. This assures your assay has the highest signal and lowest background noise.
About Regis Technologies
You can rely on the experience and quality Regis Technologies has to offer. With 50 years of service to life science industries, Regis Technologies provides unrivaled GMP synthesis and separations services and products to the pharmaceutical and biotechnology industry, and manufactures hundreds of fine organic chemicals under strict quality control specifications before release.
Does Regis sell the DNA vectors or photoproteins for coelenterazine?
No. Regis only sells the luciferase and coelenterazine bioluminescence substrates.
What can I use to dissolve coelenterazine?
Coelenterazine is easily soluble in methanol.
Which luciferases can I use with coelenterazine?
Coelenterazine can be used with Gaussia, Renilla, Obelia, Aequora, and many other marine photoproteins. Coelenterazine should not used with firefly or Vargula luciferases.
How should Regis Technologies coelenterazine be stored?
Coelenterazine should be stored at -20°C under argon or nitrogen. It can be stored as a solid or as a solution in methanol.
Can Regis manufacture coelenterazine and D-Luciferin for diagnostic device or clinical trial use?
Regis Technologies is a GMP facility and can therefore produce batches of coelenterazine under GMP conditions. Regis has experience with the FDA and other world regulatory bodies in helping to get drugs and diagnostic devices approved.
How do I order coelenterazine?
Orders can be placed by credit card either on our website or by calling Regis customer service at 847.967.6000 ext 674 or, faxing Regis customer service at +1 847 967 5876 or email@example.com.
Why buy coelenterazine from Regis Technologies?
With over 40 years of Luciferin manufacturing experience, Regis has the knowledge and flexibility to work with you to develop custom specifications, packaging and other special requests. Additionally, Regis eliminates the middle men thereby passing the savings on to you without compromising quality.