The criteria for choosing an optimal co-solvent is twofold. The first is to obtain the best suitable separation and resolution. Secondly, when scaling to a preparative separation, the ability of the co-solvent to solubilize the sample is of highest priority.

With all of the covalently bonded chiral stationary phases (CSPs) available in the market, the choice of solvents has increased. A study of various solvents will be tested on bonded and coated phases. Twelve randomly chosen samples will be run with a variety of solvents on the Whelk-O 1, RegisPack, and RegisCell CSPs.
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