Acylation reagents offer the same types of advantages available from silylation reagents: creating less polar, more volatile derivatives. However, in comparison to silylating reagents, the acylating reagents can more readily target highly polar, multi-functional compounds, such as carbohydrates and amino acids. In addition, acylating reagents offer the distinct advantage of introducing electron-capturing groups, thus enhancing detectability during analysis.

Generally, these reagents are available as acid anhydrides, acyl derivatives, or acyl halides. The acyl halides and acyl derivatives are highly reactive and may be suited for use where issues of steric hindrance may be a factor. Acid anhydrides are available in a number of fluorinated configurations, which improve detection. These fluorinated anhydride derivatives are used primarily for Electron Capture Detection (ECD), but can also be used for Flame Ionization Detection (FID). Fluorinated anhydrides are often used in derivatizing samples to confirm drugs of abuse. Despite the special utility of these reagents, their acidic nature requires that any excess or byproducts be removed prior to analysis to prevent deterioration of the column.

Download Certificates of Analysis and Safety Data Sheets online.

Email the Chromatography Department ( for more information on how acylation reagents can help with your project.