Chiral stationary phases (CSPs) prepared by coating phenylcarbamate derivatives of amylose and cellulose on supporting silica gels have proven invaluable for the separation and purification of enantiomers. One limitation of coated phases is the restricted use of certain organic mobile phases, such as acetone, chloroform, ethyl acetate, THF, and toluene. Immobilized versions have expanded the capabilities of these phases to allow for use of such solvents, though, in some cases, practitioners have observed selectivity and loading capacity differences between the immobilized and coated versions of the same chiral selector. Herein, examples of chiral separations performed using HPLC and SFC are examined with specific attention paid to sample loadability on coated and immobilized polysachharide-based CSPs.
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