The Whelk-O 1 chiral stationary phase (CSP) is useful for the separation of underivatized enantiomers in a number of families including amides, epoxides, esters, ureas, carbamates, ethers, aziridines, phosphonates, aldehydes, ketones, carboxylic acids, alcohols and non-steroidal anti-inflammatory drugs (NSAIDs). This π-electron acceptor/π-electron donor phase exhibits an extraordinary degree of generality. The broad versatility and unique selectivity of Whelk-O 1 column offers complementary separation capabilities to polysaccharide chiral stationary phases. Because Whelk-O 1 is covalently bonded to the support, the phase is compatible with all commonly used mobile phases, including aqueous systems. Other advantages include column durability, excellent efficiency, ability to invert elution order and excellent preparative loading capacity.