The global cannabis industry is growing rapidly, with many countries and US states adding regulatory frameworks for medical and recreational cannabis programs [1,2]. Quality control is an essential component in protecting the health and safety of the consumer in this emerging market, and there is increasing demand upon cannabis testing laboratories for analytical determination of multiple cannabinoids along with potential contaminants such as pesticides, mycotoxins, heavy metals, etc. Current regulations surrounding potency vary by jurisdiction, but usually require testing for the active forms of tetrahydrocannabinol (THC) and cannabidiol (CBD). In addition, many require testing for the acid forms, tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA), along with other cannabinoids like cannabigerol (CBG), cannabigerolic acid (CBGA), tetrahydrocannabivarin (THCV), cannabichromene (CBC), cannabicyclol (CBL), and cannabinol (CBN). As regulations evolve, and as research interests in minor cannabinoids expand, it is important to have robust analytical methods in place that are capable of meeting those needs.