Practical Considerations for High-Throughput Chiral Screening in HPLC and SFC with 3- and Sub-2-µm Particle-Packed Columns
In this study, we discuss and investigate some approaches to fast chiral column screening for both high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) along with the practical applicability of those approaches. Given certain constraints with instrument capabilities (e.g. maximum pressures and flow) and extra-column volume variances, we rationalize the selection of appropriate column dimensions (length [L] and inner diameter [i.d.]). We also discuss the benefits and limitations of isocratic and gradient methods for fast screening and compare experimental results obtained with columns packed with 3- and 1.6-μm particles.