The global cannabis industry is growing rapidly, with many countries and US states adding regulatory frameworks for medical and recreational cannabis programs.  Quality control is an essential component in protecting health and safety of the consumer in this emerging market and there is increasing demand upon cannabis testing laboratories for analytical determination of multiple cannabinoids. 

Current regulations concerning potency vary by jurisdiction but usually require testing for the active forms of THC and CBD.  In addition to those, many require testing for the acid forms, THCA and CBDA, along with other cannabinoids like CBG, CBGA, THCV, CBC and CBN. As regulations evolve, and as research interests in minor cannabinoids expand, it is important to have robust analytical methods in place that are capable of meeting those needs. Herein, the baseline resolution of 17 cannabinoids by high performance liquid chromatography (HPLC) with UV detection is described. Chromatographic method development was performed with attention to speed of analysis and a means for changing selectivity to improve resolution of critical pairs, as needed.

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